| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120552 |
| Chromosome: | chromosome 2 |
| Location: | 1233216 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082400 | (1 of 2) IPR000104//IPR001394//IPR028889 - Antifreeze protein, type I // Peptidase C19, ubiquitin carboxyl-terminal hydrolase // Ubiquitin specific protease domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTGTGCACCGGCAGACACGAGGGTCAA |
| Internal bar code: | ATGTCACGTCTCCGTCCAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 96.9817 |
| LEAP-Seq n confirming: | 4402 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGTCTGTGCCCTCTACT |
| Suggested primer 2: | TGCACTTTTTACGCAGCAAC |