| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120555 |
| Chromosome: | chromosome 1 |
| Location: | 603172 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003487 | (1 of 1) K13106 - pre-mRNA-splicing factor CWC26 (BUD13, CWC26) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAATGGATTCCTGTGGTGTCGGACTGTG |
| Internal bar code: | CTAGGCTCGGGGTATTGTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTGAAAGTGGGGGATGC |
| Suggested primer 2: | TGGGTCTGGGTCTGGTTAAG |