| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120556 |
| Chromosome: | chromosome 6 |
| Location: | 4346954 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278264 | (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCAGCCCCGCTGGCCCCCGCAGCTCC |
| Internal bar code: | CCCGGTGAAGTTCCAAGGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.5217 |
| LEAP-Seq n confirming: | 2913 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTTGTCAAGGATGCAGA |
| Suggested primer 2: | ATGCTGATTCGTGACAGTGC |