Insertion junction: LMJ.RY0402.120585_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.120585
Chromosome:chromosome 16
Location:5126286
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g683850 GDPD1,GDP1 Glycerophosphoryl diester phosphodiesterase family protein; (1 of 5) PTHR23344//PTHR23344:SF7 - GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE // GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATGGCATACAGAGCGACACGGAGCGAC
Internal bar code:CCGGGCGACGCGATCCGTACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:98.7746
LEAP-Seq n confirming:4675
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCCGCTTAAAGGTCTGC
Suggested primer 2:ACTAGTTCGGACCCATGCAC