Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.120629 |
Chromosome: | chromosome 14 |
Location: | 1862527 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g620500 | (1 of 6) IPR001471//IPR016177 - AP2/ERF domain // DNA-binding domain | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAATCGGACATGCAGCCGTCCACCTGCG |
Internal bar code: | ACACTCCGGTACTGTCGATTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 459 |
LEAP-Seq percent confirming: | 98.5019 |
LEAP-Seq n confirming: | 263 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGCAAAGTGATCTGGGTC |
Suggested primer 2: | CGCCTAAGCTTTGTCCTGTC |