| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120644 |
| Chromosome: | chromosome 11 |
| Location: | 2963054 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478184 | (1 of 1) 3.2.1.25 - Beta-mannosidase / Mannase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGACCTGCTGATGCGGCGGCCGCGCCCC |
| Internal bar code: | GGGCGTAACATCGGGTCTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 61.7176 |
| LEAP-Seq n confirming: | 2544 |
| LEAP-Seq n nonconfirming: | 1578 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCTGCTACTGCTGCG |
| Suggested primer 2: | CTCCTCCTTCTCCTTCCCAT |