Insertion junction: LMJ.RY0402.120674_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.120674
Chromosome:chromosome 6
Location:3520908
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278108 (1 of 5) PF13508 - Acetyltransferase (GNAT) domain (Acetyltransf_7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCTCTTCACCCCTACCTGCCAACCCGC
Internal bar code:ACGGCTAACGAACCAAAAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:657
LEAP-Seq percent confirming:98.4317
LEAP-Seq n confirming:2134
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GACGACCCAACAGGTGAACT
Suggested primer 2:GCATCCCTACACCTGCTCAT