| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120680 |
| Chromosome: | chromosome 7 |
| Location: | 4110553 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342920 | (1 of 2) 3.4.13.9 - Xaa-Pro dipeptidase / X-Pro dipeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTACAAGCCACGGCTTCACGCCCTTA |
| Internal bar code: | ATTTTCATGTGGGGCCAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 97.7186 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAACAAATACCTACGCCG |
| Suggested primer 2: | AGAGAAGGACGAGGAGGAGG |