| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120693 |
| Chromosome: | chromosome 1 |
| Location: | 5251779 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036650 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCTCAAACGCCTCCACCGGATGTGTG |
| Internal bar code: | GCATGCACAACCCGATAGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.7251 |
| LEAP-Seq n confirming: | 1814 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGAGTGCCTGGCTTACC |
| Suggested primer 2: | CCCAGATTGCAAACCTGATT |