| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120721 |
| Chromosome: | chromosome 1 |
| Location: | 3877391 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025200 | MMP4 | Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGAAAGCAGAAAGCCCGACAAATGAA |
| Internal bar code: | GTGGGCACCGCGGGAGAGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 96.9474 |
| LEAP-Seq n confirming: | 921 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTGAATCATGACCCCAC |
| Suggested primer 2: | ACAAGGGGACACTTTGTTGC |