Insertion junction: LMJ.RY0402.120747_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.120747
Chromosome:chromosome_3
Location:3990666
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g171900 FAP56 Flagellar Associated Protein sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTTCTGGACTCGTGCGGACGGCGTGTGACA
Internal bar code:GCATCGTAGGTTCTAGGGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:99.8134
LEAP-Seq n confirming:4280
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACTTGTATCAGCGGCAG
Suggested primer 2:AGCTGTTATTTCCCCACACG