| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120771 |
| Chromosome: | chromosome 12 |
| Location: | 3305913 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGGGTGGTCCGCACCCCGAACCCCGC |
| Internal bar code: | CCTCTGTATGGGCACCGGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 97.0687 |
| LEAP-Seq n confirming: | 1159 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGTAGACGCCGAGTTC |
| Suggested primer 2: | GGGAGCCAATGTTGATGACT |