Insertion junction: LMJ.RY0402.120790_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.120790
Chromosome:chromosome 3
Location:906087
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g146727 GOX9 Glyoxal oxidase 9; (1 of 2) IPR009880//IPR011043//IPR013783//IPR014756//IPR015202//IPR015916 - Glyoxal oxidase, N-terminal // Galactose oxidase/kelch, beta-propeller // Immunoglobulin-like fold // Immunoglobulin E-set // Domain of unknown function DUF1929 // Galactose oxidase, beta-propeller intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCATTCAAACGTCCAGCAGATTCCAAGAT
Internal bar code:ACCTCGCTCAGCAGGGCCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:729
LEAP-Seq percent confirming:99.1685
LEAP-Seq n confirming:1789
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGCGACCGTGATCTCTAC
Suggested primer 2:CAACAAACCACAGGATGTCG