| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120792 |
| Chromosome: | chromosome_11 |
| Location: | 328235 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467567 | UBQ1 | Ubiquitin | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTGCGTTCATTGCCGCTTCCTGCGCCCGC |
| Internal bar code: | GACAACCGACATCTTAATCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 61.5478 |
| LEAP-Seq n confirming: | 509 |
| LEAP-Seq n nonconfirming: | 318 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCCAGGACAAGGAGGGT |
| Suggested primer 2: | CCTACCCCGCCTAAGAAAAG |