Insertion junction: LMJ.RY0402.120812_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.120812
Chromosome:chromosome 14
Location:1162697
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g615700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCTGGCACTATGGCGCGCGCAGCGGACC
Internal bar code:GTGCCACGAAAGGTTATCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:407
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCCCAGATCTTTTCC
Suggested primer 2:ATCCCCAAACCGTGTGTCTA