| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120830 |
| Chromosome: | chromosome 12 |
| Location: | 8327557 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549600 | (1 of 96) IPR019734 - Tetratricopeptide repeat | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAATTAAATCATTGCAAACCGGTGTGCGT |
| Internal bar code: | GATCTAACTAATTAATGGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.5434 |
| LEAP-Seq n confirming: | 7630 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGTCAGGGAGGTTGTA |
| Suggested primer 2: | CACTTCCTGCCCTCAGCTAC |