| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120864 |
| Chromosome: | chromosome 16 |
| Location: | 165954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695100 | ACX4 | (1 of 1) PTHR10909//PTHR10909:SF331 - ELECTRON TRANSPORT OXIDOREDUCTASE // ACYL-COENZYME A OXIDASE 4, PEROXISOMAL; Putative Acyl-CoA oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGGAATGCGCCGTGCAGGGGGGGGGG |
| Internal bar code: | AATGTGGCTGACACTCCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 91.7969 |
| LEAP-Seq n confirming: | 235 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCACAATCCTTCAATGG |
| Suggested primer 2: | TTTTACGGCTTTGGGTGAAG |