| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120881 |
| Chromosome: | chromosome_12 |
| Location: | 4415756 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g520950 | BOP5,DIC4,IC138 | Flagellar inner dynein arm I1 intermediate chain IC138 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCGTGTCCATCGCCACCGTCACGCCTGC |
| Internal bar code: | GATCAACCCCCCTGATTCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGTAGCTGTTGTGACCC |
| Suggested primer 2: | AGCGAGGCGGACTATGACTA |