| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120898 |
| Chromosome: | chromosome 17 |
| Location: | 747713 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701300 | (1 of 1) K17491 - protein phosphatase 4 regulatory subunit 3 (SMEK, PPP4R3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGCAAGCGGATGCGTAGCGAGGACGG |
| Internal bar code: | CGGACCCGTCGCAGGCGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.8552 |
| LEAP-Seq n confirming: | 1379 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTACTTCAGCGAGGACGC |
| Suggested primer 2: | ACAGTAATTTGGATTCGGCG |