Insertion junction: LMJ.RY0402.120901_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.120901
Chromosome:chromosome 4
Location:492550
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGTGAGTCAAGAATTTAAGTTTCGGGG
Internal bar code:TGGGTAGGTCCGTTTTTCTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:99.637
LEAP-Seq n confirming:3019
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTGTGTGTGTGTGTGT
Suggested primer 2:CCATAATCAGGGTCCACCAC