| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120912 |
| Chromosome: | chromosome 1 |
| Location: | 2458598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013801 | SXD1,TCY1 | (1 of 1) 5.5.1.24 - Tocopherol cyclase; Tocopherol cyclase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGGGGGTGAGGTGGCAAGGGGGCGCAG |
| Internal bar code: | CTAGCGGCTCTCGGGGGGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 95.396 |
| LEAP-Seq n confirming: | 4517 |
| LEAP-Seq n nonconfirming: | 218 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAGCTGACCACTTTCAT |
| Suggested primer 2: | TCCTGGAACTCATACCTGGC |