| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120913 |
| Chromosome: | chromosome 14 |
| Location: | 75160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGCGACGCCGCTCCCGACAAGCCCCCT |
| Internal bar code: | ACCCAGACGATAGGGTATCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 84.6405 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGTGTCCTTCTCCTGCG |
| Suggested primer 2: | GCGGTACCCTTGAACCTGTA |