| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120915 |
| Chromosome: | chromosome_11 |
| Location: | 3632090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g482200 | FBB9 | Flagellar/basal body protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGATGCGAAGCGGGGCCGCGGGTTTGGAGG |
| Internal bar code: | ACGCTGGCGCCAGGCGACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 99.8743 |
| LEAP-Seq n confirming: | 3973 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCGGGCTACATTGACT |
| Suggested primer 2: | CACAGAGTCAGGCAACTCCA |