| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120927 |
| Chromosome: | chromosome 6 |
| Location: | 8768141 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309951 | (1 of 1) 3.2.1.18 - Exo-alpha-sialidase / Sialidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCATCCCGATCGTAGTTGCGAAGCAGTG |
| Internal bar code: | AGCTTCTTGGAGTAAACATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 792 |
| LEAP-Seq percent confirming: | 99.6236 |
| LEAP-Seq n confirming: | 1588 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGTGAACATGCAACCA |
| Suggested primer 2: | TGTAAAGCAATGCGAGATGC |