| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120929 |
| Chromosome: | chromosome 9 |
| Location: | 915035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401150 | NUOFAF2 | (1 of 1) K18160 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 (NDUFAF2); NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGAGCTCACGTCTCAGCGAGACAAAC |
| Internal bar code: | GTCTGTTAGCCCGCATGGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 98.8142 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGCTACGAAGAGGTCG |
| Suggested primer 2: | GTTTGGAGGGTTGTGCAAGT |