| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120938 |
| Chromosome: | chromosome 6 |
| Location: | 6893582 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295700 | MCM3 | (1 of 1) K02541 - DNA replication licensing factor MCM3 (MCM3); Minichromosome maintenance protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGGAAATGGTTCCAATGGGCTCCTTC |
| Internal bar code: | AGTTTTCGCCGTGTCTATTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAAATGAAAGGGGGCTC |
| Suggested primer 2: | CAGGCCGATGTAAAGGTTGT |