Insertion junction: LMJ.RY0402.120945_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.120945
Chromosome:chromosome 17
Location:2723473
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g718150 Glycosyl transferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTCTTGAGGCGAGTTTGTGCGTTACCGC
Internal bar code:AGTTTTAGTAGCCCCAGTCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:696
LEAP-Seq percent confirming:99.1842
LEAP-Seq n confirming:851
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAAGAAGACCAGAACCTG
Suggested primer 2:TTTTGCAGTGACAGACTCGG