| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120945 |
| Chromosome: | chromosome 17 |
| Location: | 2723480 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718150 | Glycosyl transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTAGGCACGCGGATCAGTGTGATGGAC |
| Internal bar code: | GACCAAACCTCAGATCTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 99.0233 |
| LEAP-Seq n confirming: | 3650 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAGAAGACCAGAACCTG |
| Suggested primer 2: | TTTTGCAGTGACAGACTCGG |