| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120949 |
| Chromosome: | chromosome_12 |
| Location: | 4009833 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g516950 | SAS6 | Basal body cartwheel protein | sense | CDS/intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTCGGAGCTCAGTCACAAGCTGGGTAGC |
| Internal bar code: | GGATATGACGCGTCGCCGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 99.6091 |
| LEAP-Seq n confirming: | 1274 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCCAAACAGGATGACCG |
| Suggested primer 2: | AGCGTTCATCCTCTCATGCT |