| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120962 |
| Chromosome: | chromosome_1 |
| Location: | 3553045 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g022750 | FAP159 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCATAGGTTTCCTGTTCCTCGTCGGGGTC |
| Internal bar code: | GAACGTATGGATCTGGGCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 95.8297 |
| LEAP-Seq n confirming: | 1103 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGCAGGACAGGAACAC |
| Suggested primer 2: | CATGAGTTAGGCGTTGGGTT |