| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120969 |
| Chromosome: | chromosome_1 |
| Location: | 2367839 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g012950 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGATTGGGCGTCGGTAGCGGTGCCTGAT |
| Internal bar code: | TTGCAGACCCGTGGCCAGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.4987 |
| LEAP-Seq n confirming: | 1985 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGAACTCGATACCAAA |
| Suggested primer 2: | TGGTGGTGAGGGTAGTGACA |