Insertion junction: LMJ.RY0402.120987_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.120987
Chromosome:chromosome 11
Location:1116315
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467678 COP6,HKR2 (1 of 5) IPR001660//IPR013761 - Sterile alpha motif domain // Sterile alpha motif/pointed domain; Histidine cyclase rhodopsin 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAATAATTTCTATCATTTAGGTGCTGTG
Internal bar code:GATCGTGTACTTACTCGCGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:664
LEAP-Seq percent confirming:99.4024
LEAP-Seq n confirming:998
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCAGGAGGGAACCATCT
Suggested primer 2:GGTACTGTGATGCTTCGGGT