Insertion junction: LMJ.RY0402.121006_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.121006
Chromosome:chromosome 6
Location:1748469
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g261950 ANK11 (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4); Predicted protein with ankyrin repeats 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTGCGGTCGTGCAGGTTGAAGCGGCGA
Internal bar code:ATGCGACTCTAGAGAAACCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:753
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACGAGGGACTGAAAAACCA
Suggested primer 2:AGGTGTAATGTCGCCTCACC