Insertion junction: LMJ.RY0402.121008_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.121008
Chromosome:chromosome 6
Location:6432466
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g292500 CYN23b,CYN23 Cyclophilin 23; (1 of 1) PTHR11071//PTHR11071:SF17 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTGCAACCGTTTGAGCCGTTGAGACAC
Internal bar code:CACGGGATCACATCACGCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:799
LEAP-Seq percent confirming:99.7163
LEAP-Seq n confirming:1406
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAAGCTTCCCTCAACTT
Suggested primer 2:CCCACACACACACACAGACA