| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121031 |
| Chromosome: | chromosome 5 |
| Location: | 1252068 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245352 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCGAGGGGCTGGGCGGGGAGGGGCTA |
| Internal bar code: | CAAAGCCCAGGGGCCATGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 96.2357 |
| LEAP-Seq n confirming: | 588 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCGCTCACATGAAAAA |
| Suggested primer 2: | GCTGGTGTGATCGGTTACCT |