| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121033 |
| Chromosome: | chromosome 12 |
| Location: | 6270123 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537100 | TSV,TSV2 | Valyl-tRNA synthetase; (1 of 1) PTHR11946//PTHR11946:SF5 - ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES // VALYL-TRNA SYNTHETASE, ISOFORM A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCTCGGCGTAGCCGGGCACGTTCATCA |
| Internal bar code: | TTGGTGCGATAGGTCTATGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 99.8031 |
| LEAP-Seq n confirming: | 2028 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTGGTGAAGGTGTGAG |
| Suggested primer 2: | GCGACATTAAGGATGGCATT |