| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121039 |
| Chromosome: | chromosome 5 |
| Location: | 3069585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238935 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGAGCAGGTGACAGATCGAACGCTTAT |
| Internal bar code: | ACCCGGGGGGTACAACCAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 95.6358 |
| LEAP-Seq n confirming: | 4076 |
| LEAP-Seq n nonconfirming: | 186 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCATCGAAGACACGTCG |
| Suggested primer 2: | GGGCTATTTGACGATGAGGA |