Insertion junction: LMJ.RY0402.121042_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.121042
Chromosome:chromosome 7
Location:3679214
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g337450 GT90F9,GT90-9,CGL75 Glycosyl transferase GT90 family protein 9; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGTGTTAAGGAACCGTGCCGTGAACGCC
Internal bar code:ATCTCCCCGAGCCAGTTGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:505
LEAP-Seq percent confirming:9.30233
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTACGGTGGATGCGTAAG
Suggested primer 2:AGACCGGTGACACTTGGTTC