| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121054 |
| Chromosome: | chromosome_2 |
| Location: | 5220830 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105950 | FAP174 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTGCTCAAGGCTATCCACCAAATATCTTA |
| Internal bar code: | TCTTTGGGTTGTGTGTTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1204 |
| LEAP-Seq percent confirming: | 99.5591 |
| LEAP-Seq n confirming: | 6774 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCCTACAGAGACTTGGAC |
| Suggested primer 2: | ATGGAAGCATTTTGCAATCC |