Insertion junction: LMJ.RY0402.121087_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.121087
Chromosome:chromosome 10
Location:2396139
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g435350 ESU1,ESU1a Endosulfine cAMP-regulated phosphoprotein; (1 of 2) PF04667 - cAMP-regulated phosphoprotein/endosulfine conserved region (Endosulfine) 5'UTR|CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACGCCCAAATGGCCGCGACAGAAGCA
Internal bar code:TCCCAAGAATCAATTATCTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:596
LEAP-Seq percent confirming:99.646
LEAP-Seq n confirming:563
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCCAAGAAGTTGAGACC
Suggested primer 2:TTTGCTATCAGTGCGTCCAG