| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121087 |
| Chromosome: | chromosome_10 |
| Location: | 2396139 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g435350 | ESU1 | Endosulfine cAMP-regulated phosphoprotein | sense | 5'UTR|CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGGACGCCCAAATGGCCGCGACAGAAGCA |
| Internal bar code: | TCCCAAGAATCAATTATCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 99.646 |
| LEAP-Seq n confirming: | 563 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCCAAGAAGTTGAGACC |
| Suggested primer 2: | TTTGCTATCAGTGCGTCCAG |