Insertion junction: LMJ.RY0402.121135_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.121135
Chromosome:chromosome 6
Location:361779
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g251650 PTC1 (1 of 1) K14430 - phosphate transporter (PHO87_91); Low-affinity phosphate transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCCGCCGGCCTGCCCCCGCCCCTGCCG
Internal bar code:ACACGTTAACTGGGCCCCGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:56
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTAATGAAACGCC
Suggested primer 2:ATTTGCTCCCGTCCCTAACT