| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121189 |
| Chromosome: | chromosome 3 |
| Location: | 4485950 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176150 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTATATGTATGTTTTGGATGCGTCGGCG |
| Internal bar code: | GGGAATTTCGATATAGTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 99.6616 |
| LEAP-Seq n confirming: | 5007 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGACACACAAATTGCAC |
| Suggested primer 2: | TCATCAGGTTGTAACGCACC |