Insertion junction: LMJ.RY0402.121201_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.121201
Chromosome:chromosome 10
Location:2843670
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g439650 COG8 (1 of 1) PF04124 - Dor1-like family (Dor1); Component of oligomeric golgi complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCCAGCGCATCATGGAAAGGGAAGCGT
Internal bar code:TGCGCGAACGGTCATACGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:262
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:756
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CATTTGGCACGTAGGGAACT
Suggested primer 2:AGTGCTGTGATGCAAAGGTG