| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121207 |
| Chromosome: | chromosome 10 |
| Location: | 1341487 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427800 | (1 of 3) IPR002889//IPR013994 - Carbohydrate-binding WSC // Carbohydrate-binding WSC, subgroup | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACGCAGAGGCTCCCCGAGCCGGAGGCC |
| Internal bar code: | TCCTGGAGGGTGGGTCGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGCGAGGGAAAAGATAC |
| Suggested primer 2: | TCAGTATAGTCTGCGGCGTG |