| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.121213 |
| Chromosome: | chromosome 16 |
| Location: | 1773417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655050 | NON1 | NO-nitrate 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGGATGCCGGTGGCGGAGGGCGGCGC |
| Internal bar code: | GGGCCCTCTTCGGTCCTGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 334 |
| LEAP-Seq percent confirming: | 99.8542 |
| LEAP-Seq n confirming: | 685 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGAGGTGGTAAAAGACG |
| Suggested primer 2: | AGCTCAGTCCTCGCTCACTC |