| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.121215 |
| Chromosome: | chromosome 11 |
| Location: | 1475717 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467740 | (1 of 1) PTHR10108//PTHR10108:SF705 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTGTCGCGCTGCATCTGCCACCCACCG |
| Internal bar code: | TGGGGACATCCGAGCAAAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 63.9443 |
| LEAP-Seq n confirming: | 1699 |
| LEAP-Seq n nonconfirming: | 958 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACAGTCCAGCGCGAAAT |
| Suggested primer 2: | TCCCCGGAAAGTTGTAGTTG |