Insertion junction: LMJ.RY0402.121246_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.121246
Chromosome:chromosome 11
Location:3444856
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g481050 (1 of 10) PF00505 - HMG (high mobility group) box (HMG_box); High mobility group protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTATTATGAACTATTGTATGTTTGAGGG
Internal bar code:CCCCTAGCAACTGTGTCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:567
LEAP-Seq percent confirming:95.7118
LEAP-Seq n confirming:2165
LEAP-Seq n nonconfirming:97
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCACACGTTACATCAATC
Suggested primer 2:CCACTACAGCAGGGACAGGT