| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121257 |
| Chromosome: | chromosome 12 |
| Location: | 7456379 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556300 | CFAP300,C11orf70,FBB5 | Flagellar basal body proteome 5 / Dynein assembly factor; (1 of 1) PF14926 - Domain of unknown function (DUF4498) (DUF4498) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAATGCACCATGAATGCGGATGTTACAG |
| Internal bar code: | CTTGAATGGTATGCTTCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 92.7435 |
| LEAP-Seq n confirming: | 23606 |
| LEAP-Seq n nonconfirming: | 1847 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGATTCGTCGTACAGGGTC |
| Suggested primer 2: | AGAAAACACGGCCCATACAC |