| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.121262 |
| Chromosome: | chromosome 17 |
| Location: | 2107295 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712300 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTGTCATCCTCCTGCGTCGGCAGCTC |
| Internal bar code: | TAGTAAATGCTCTCAAATTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 400 |
| LEAP-Seq percent confirming: | 99.7682 |
| LEAP-Seq n confirming: | 5594 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAAGAGAAGGTGCAGAT |
| Suggested primer 2: | TCATGTAGCCAGCAGCAAAC |