| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.121262 |
| Chromosome: | chromosome 17 |
| Location: | 2107323 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712300 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTTCACGCGCCTATGCCCCTCTTGTAC |
| Internal bar code: | CGCCGCCCAAACAGACGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 150 |
| LEAP-Seq percent confirming: | 2.54873 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 2600 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAAGAGAAGGTGCAGAT |
| Suggested primer 2: | TCATGTAGCCAGCAGCAAAC |